Nom du corpus

Corpus Systématique Végétale

Titre du document

The extraction and assay of soil trehalase

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Biological activity
  • Disaccharides
  • Hydrolytic enzymes
  • Methodology Soil enzymes
  • Soil organic matter
  • Trehalose l-glucohydrolase E.C. 3.21.28
Nom du fichier dans la ressource
Syst_veg6_v2_009647
Auteur(s)
  • R. E. Smith 1
  • R. Rodriguez-Kabana 2
Affiliation(s)
  • 1) Great Lakes Chemical Corporation, 47906, West Lafayette, Indiand
  • 2) Department of Botany, Plant Pathology, and Microbiology, Auburn University, Agricultural Experiment Station, 36849, Auburn, Alabama, USA
Résumé

Trehalase is a specific enzyme for the hydrolysis of trehalose, a storage carbohydrate of insect and microbial species. The enzyme is of rare occurrence among higher plants. In cultivated soil, trehalase activity (Ta) was linearly related to both the amount of soil and assay incubation time. Ta increased sharply in response to substrate concentration over the range of 0 to 2% (w/v); higher substrate levels, however, showed a reduced rate of increase. Soil trehalase activity increased proportionally with increased incubation temperature over the range of 20 to 50°C but declined sharply at temperatures above 50°C. Ta was maximal at pH 5.0 when 0.5M acetate or propionate buffers were used; however, activity diminished with increased ionic strength of the buffer. Based on these findings, a standard assay method for Ta was developed. The enzyme was extracted from soil and eluted from a Sephadex G 200 column as high molecular weight organic matter. Treatment of the extract with beta-glucosidase reduced the molecular weights of the elution fractions exhibiting Ta.

Catégories Science-Metrix
  • 1 - applied sciences
  • 2 - agriculture, fisheries & forestry
  • 3 - agronomy & agriculture
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Plant Science
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Soil Science
Catégories WoS
  • 1 - science ; 2 - soil science
  • 1 - science ; 2 - plant sciences
  • 1 - science ; 2 - agronomy
Identifiant ISTEX
BD32BEB88F94B4A4134D27090336D6CB2A9B3D86
Revue

Plant and Soil

Année de publication
1982
Présence de XML structuré
Non
Score qualité du texte
7.417
Version PDF
1.3
Type de publication
Journal
ark:/67375/1BB-02PR7BTV-K
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