Nom du corpus

Corpus Systématique Végétale

Titre du document

Molecular cloning and characterization of novel isoforms of potato ADP-glucose pyrophosphorylase

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • ADP-glucose pyrophosphorylase (large subunit)
  • Potato ( Solanum tuberosum L.) Starch synthesis
Nom du fichier dans la ressource
Syst_veg6_v2_022682
Auteur(s)
  • Ursula La Cognata 1
  • Lothar Willmitzer 1
  • Bernd Müller-Röber 1
Affiliation(s)
  • 1) Institut für Genbiologische Forschung Berlin GmbH, Ihnestraße 63, 14195, Berlin, Germany
Résumé

ADP-glucose pyrophosphorylase (AGPase) is one of the major enzymes involved in starch biosynthesis in higher plants. We report here the molecular cloning of two cDNAs encoding so far uncharacterized isoforms (AGP S2 and AGP S3) of the potato enzyme. Sequence analysis shows that the two polypeptides are more homologous to previously identified large subunit polypeptides from potato and other plant species than to small subunit isoforms. This observation suggests that AGP S2 and AGP S3 represent novel large subunit polypeptides. agpS2 is expressed in several tissues of the potato plant, including leaves and tubers. Expression was stronger in sink leaves than in source leaves, indicating developmental regulation. In leaves, agpS2 expression was induced 2- to 3-fold by exogenous sucrose; therefore, agpS2 represents a new sucrose-responsive gene of starch metabolism. Expression of agpS3 was restricted to tubers: no agpS3 expression could be seen in leaves of different developmental stages, or when leaves were incubated in sucrose. Therefore, agpS3 represents the only AGPase gene so far characterized from potato, which is not expressed in leaves. Conversely, all four AGPase isoforms known from potato are expressed in tubers.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - genetics & heredity
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - physiologie vegetale et developpement
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
Identifiant ISTEX
669F7C929BE82BD7454E0F158F5BAABA724011F1
Revue

Molecular and General Genetics MGG

Année de publication
1995
Présence de XML structuré
Non
Score qualité du texte
9.232
Version PDF
1.3
Type de publication
Journal
ark:/67375/1BB-03D85QS3-K
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