Nom du corpus

Corpus Systématique Végétale

Titre du document

In vitro polyploidization of mature ostrich fern sporophytes through rejuvenation

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Apospory
  • diploid
  • gametophyte
  • rejuvenation
  • sporophyte
  • tetraploid
Nom du fichier dans la ressource
Syst_veg6_v2_023311
Auteur(s)
  • Doug M. Materi 1
  • Leo A. Dionne 1
  • Bruce G. Cumming 1
Affiliation(s)
  • 1) Department of Biology, University of New Brunswick, College Hill, Box 4400, E3B 5A3, Fredericton, New Brunswick, Canada
Résumé

An in vitro method is described for producing ostrich fern (Matteuccia struthiopteris (L.) Todaro) polyploids from mature sporophytes as a possible means of plant improvement in this economically important fern species. The procedure is based on rejuvenating adult sporophytes (2n) to enable the aposporous production of diploid (2n) gametophytes, and then mating the gametophytes to produce tetraploid (4n) sporophytes. The adult sporophytes were rejuvenated by culturing excised shoot tips for a minimum of three months in a liquid medium (Murashige and Skoog salts) under conditions of extreme carbohydrate deprivation (0.01% sucrose). Apospory was induced by culturing leaves excised from the rejuvenated shoots for two months on a semi-solid medium lacking sucrose, resulting in the production of diploid gametophytes. The gametophytes were transferred to fresh medium and grown to sexual maturity for one or two months, then floated on the surface of a liquid medium containing 0.01% sucrose for up to two months to promote opening of the sex organs. Subsequent self-fertilization resulted in the successful production of tetraploid sporophytes in 11 of the 14 diploid clones in which polyploidization was attempted. Tetraploids (4n=156) were confirmed by cytological examination. This method permits polyploidization of mature, fully characterized plants.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - plant biology & botany
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - invertebres
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Horticulture
Catégories WoS
  • 1 - science ; 2 - plant sciences
  • 1 - science ; 2 - biotechnology & applied microbiology
Identifiant ISTEX
35CC8747B00BF04D92EBE6F740C80AD2707D5CBC
Revue

Plant Cell, Tissue and Organ Culture

Année de publication
1995
Présence de XML structuré
Non
Score qualité du texte
6.755
Version PDF
1.3
Type de publication
Journal
ark:/67375/1BB-04HLSV1F-X
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