Nom du corpus

Corpus Systématique Végétale

Titre du document

Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • eucalyptus
  • wood
  • xylem
  • lignin biosynthesis
  • cinnamyl alcohol dehydrogenase
  • promoter-GUS fusion
Nom du fichier dans la ressource
Syst_veg6_v2_028508
Auteur(s)
  • Catherine Feuillet 1
  • Virginie Lauvergeat 1
  • Christine Deswarte 1
  • Gilles Pilate 2
  • Alain Boudet 1
  • Jacqueline Grima-Pettenati 1
Affiliation(s)
  • 1) Centre de Biologie et Physiologie Végétales, URA CNRS 1457, Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse cédex, France
  • 2) INRA Centre d'Orléans Ardon, 45160, Orléans, France
Résumé

Cinnamyl alcohol dehydrogenase (CAD) which catalyses the synthesis of the cinnamyl alcohols, the immediate precursors of lignins, from the corresponding cinnamaldehydes is considered to be a highly specific marker for lignification We have isolated and characterized a CAD genomic clone from eucalyptus, a woody species of economic importance. The full-length promoter (EuCAD, 2.5 kb) and a series of 5′ deletions were fused to the β-glucuronidase (GUS) reporter gene. These constructs were tested in a homologous transient expression system of eucalyptus protoplasts which enabled the identification of several regions involved in transcriptional control. In order to study the spatial and developmental regulation of the CAD gene, the chimeric gene fusion (EuCAD-GUS) was then transferred via Agrobacterium tumefaciens-mediated transformation into poplar, an easily transformable woody angiosperm. Quantitative fluorometric assays conducted on eight independent in vitro transformants showed that GUS activity was highest in roots followed thereafter by stems and leaves. Histochemical staining for GUS activity on both in vitro primary transformants and more mature greenhouse-grown plants indicated a specific expression in the vascular tissues of stems, roots, petioles and leaves. At the onset of xylem differentiation, GUS activity was detected in parenchyma cells differentiating between the xylem-conducting elements. After secondary growth has occurred, GUS activity was localized in xylem ray cells and parenchyma cells surrounding the lignified phloem and sclerenchyma fibers. This first characterization of a woody angiosperm CAD promoter provides functional evidence for the role of CAD in lignification and suggests that parenchyma cells expressing CAD may provide lignin precursors to the adjacent lignified elements (vessels and fibres).

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - plant biology & botany
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Plant Science
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Agronomy and Crop Science
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - plant sciences
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
BC2C4676ADFBBBE55A6B2A34B72116B24724505B
Revue

Plant Molecular Biology

Année de publication
1995
Présence de XML structuré
Non
Score qualité du texte
10
Version PDF
1.3
Type de publication
Journal
ark:/67375/1BB-09W7QQ2S-7
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