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Corpus Systématique Végétale

Titre du document

The levels of two distinct species of phytochrome are regulated differently during germination in Avena sativa L.

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Springer (journals)
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Mots-clés d'auteur
  • Avena (phytochrome)
  • Enzyme-linked immunosorbent assay (ELISA)
  • Phytochrome from green tissue
  • Seed germination
  • ELISA : enzyme-linked immunosorbent assay
  • Ig : immunoglobulin
  • kDa : kilodalton
  • Pfr, Pr : far-red-absorbing and red-absorbing forms of phytochrome, respectively
  • SDS-PAGE : sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Nom du fichier dans la ressource
  • James G. Tokuhisa 1,2
  • Peter H. Quail 1,2
  • 1) Department of Botany, University of Wisconsin, 53706, Madison, WI, USA
  • 2) Department of Genetics, University of Wisconsin, 53706, Madison, WI, USA

The abundance and molecular mass of phytochrome in germinating embryos of A. sativa (oat) grown in light or darkness have been monitored using immunoblot and spectrophotometric assays. Immunoblot analysis shows that imbibed but quiescent embryos have two immunochemically distinct species of phytochrome with monomeric molecular masses of 124 and 118 kDa (kdalton). The 118-kDa species has the properties of the 118-kDa phytochrome extracted from fully green oat tissue (J.G. Tokuhisa, S.M. Daniels, P.H. Quail, 1985, Planta 164, 321–332), whereas the 124-kDa polypeptide appears similar to the well-characterized photoreceptor of etiolated tissue. The capacity of antibodies directed against etiolated-oat phytochrome to immunoprecipitate the 124-kDa species but not the 118-kDa species has been exploited to quantitate the levels of each separately over a 72-h time course of germination and seedling development. The abundance of the 124-kDa molecule increases at least 200-fold in etiolated seedlings over 72 h whereas in light-grown seedlings the level of this molecule is relatively constant. In contrast, the amount of the 118-kDa species increases only twofold in both dark- and light-grown seedlings over the same period of time. These data indicate that whereas the abundance of 124-kDa phytochrome is regulated at the protein level by the well-documented, differential stability of the red- and far-red-absorbing forms in vivo, the 118-kDa molecule is present at a low constitutive level, presumably reflecting no such difference in the stability of the two spectral forms.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - plant biology & botany
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Plant Science
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - plant sciences
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