Nom du corpus

Corpus Systématique Végétale

Titre du document

Long-term optimized embryogenic cultures in durum wheat ( Triticum durum Desf.)

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Syst_veg6_v2_002511
Auteur(s)
  • G. M. Borrelli 1
  • E. Lupotto 2
  • F. Locatelli 2
  • G. Wittmer 1
Affiliation(s)
  • 1) Istituto Sperimentale per la Cerealicoltura, S.S. 16 Km, 675 — 71100, Foggia, Italy
  • 2) Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy
Résumé

Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum of immature embryos explanted in the presence of 2,4 dichlorophenoxyacetic acid. Selective subculture of the white, compact, embryogenic sectors led to the establishment of long-term cultures. Regeneration occurred on hormone-free medium either via germination of somatic embryos, or via multiple-shoot formation probably due to precocious germination of somatic embryos. The three varieties, Ofanto, Creso and Appulo, were the best responding genotypes. Callus fragmentation and two subsequent transfers onto fresh medium at 7-day intervals yielded a frequency of plant regeneration of some 25–40 plantlets per gram fresh weight callus in 21 days on Murashige and Skoog's hormone-free medium. Plantlets could be efficiently established in soil, thus confirming the possibility of biotechnological approaches with varieties of this crop species.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - plant biology & botany
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - agronomie. sciences du sol et productions vegetales
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Plant Science
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Agronomy and Crop Science
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - plant sciences
Identifiant ISTEX
13D9776D86084D512D85D5D1FD43BE4455033A5D
Revue

Plant Cell Reports

Année de publication
1991
Présence de XML structuré
Non
Score qualité du texte
6.565
Version PDF
1.3
Type de publication
Journal
ark:/67375/1BB-0PNJ17ML-G
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